Thanks! I rescanned using grayscale and it opened up as 16-bit in imagej
with the different tones :) Now, however, I have another problem. I
converted the image into red, green, and blue components once (it had it set
up so it was all in the same window and had a scroller at the bottom) and
can't figure out how to do it again. Are there any suggestions on how I can
extract the red component of the image by converting it to a stack or in any
other way?
On Wed, 11 Jun 2008 14:35:11 -0500, John Alexander <
[hidden email]>
wrote:
>I'm just guessing here - but the 16-bit transmissive mode is a black and
>white image.
>
>The reflective mode is probably scanning in color - which is only going
>to be 8-bit for the red, green, blue channels.
>
>Re-scan as a black and white image - and then you should get 16-bit depth.
>
>I don't think you will get a 16-bit red, green, blue color image - that
>would be a 48-bit per pixel image.
>
>
>
>Lisa wrote:
>> I have some files saved as 16-bit, but when I open them in imagej there are
>> only 256 different tones shown as I scroll over the image. I have a scanner
>> which has two scanning beds - reflection and transmission. The images I have
>> scanned using the transmission mode at 1200dpi and saved as 16 bit show the
>> 65536 different tones, but the images I scanned in reflection mode at 300dpi
>> and saved as 16 bit only show 256 different tones. I am decomposing the
>> images such that I am only working with the red part. I am new to the
>> program and to the scanner, so I am unsure what I could be doing
>> incorrectly. Thanks!
>>
>
>--
>John K. Alexander, Ph.D.
>Post-Doctoral Fellow
>William Green Laboratory
>University of Chicago
>Dept. Neurobiology, Pharmacology, and Physiology
>947 East 58th Street
>Abott Hall 402
>Chicago, IL 60637
>(off) 773-702-9386
>(fax) 773-702-3774
>
[hidden email]
>=========================================================================
Locked
