16-bit signed images

[Zummy]

Is there a good way to pass 16-bit signed images to ImageJ
from a Java program.  I have a short[] array that I would
like to send.  There are ShortProcessor constructors
that have a boolean unsigned option, but the they are
obsolete.  We are background subtracting the images, and
would REALLY like to see negative values.  Right now, they
are coming out all white.

We have another viewer, so this is not time critical, it would
just be very nice to have.  We are aware of the potential to
exceed a 15-bit quantity--that shouldn't happen very often.

Thanks,

John

[Leslie Day]

Any tricks for getting accurate particle counting in low contrast images?

Leslie Day
Biology Department
Northeastern University
[hidden email]
617-373-3780

[Andrew Kligerman]

Are these color or B x W images? If color you can try splitting the
images into their component colors and look for the ones with better
contrast.  You can also employ digital filters to enhance contrast.

Andrew Kligerman
USEPA

[Ted Ballou]

I often have good results with the "Pseudo-flat field" filter -
increases the contrast of dye-injected neuronal dendrites, and partially
removes the quilting effect in montaged fluorescent images.

Ted Ballou
Northwestern U Department of Physiology