Hi Julieta,
I think you could use the TrakEM2 plugin for your 2-D gels. It was
originally meant for EM-images but it has lots of options for aligning
different image layers in a stack and mixing them. There are enough
online tutorials around to get you started. I say this without knowing
those other software solutions and what they can do. Probably, TrakEM2
would lack the more specialized analysis tools in comparison.
Hope this helps
Christian
---
Christian Goosmann
Mikroskopie
Max-Planck-Institut für Infektionsbiologie
Campus Charité Mitte
Charitéplatz 1
10117 Berlin
Tel.: +49 30 28460 388
juli goenaga wrote:
> Hello all,
>
> I'm trying to used Image J to analyze 2-dimentional gels with the goal of identifying spots or proteins that could show different expresion in a group of samples. This is the tipical analysis of proteomic profile that you can do with software as Melanie, Delta2D, SameSpots.
> However, it is difficult to perform a proper analysis and I am still not sure if I can use Image J for the analysis of 2D-gels.
>
> I would like to know if some of you are using this software for proteomics studies. Or if you already know that it is impossible to use Image J for this.
> I really appreciate any comments and contribution.
>
>
> Thank you very much for your time!
> Kind regards,
> Julieta
>
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