32-bit images to 16-bit images

[Peter Mc]

Hi everyone;

Performing a few conversions from 32-bits real images stack to 16 - bit images stack, I just realized that each frame is being scaled from 0 to 65535 so guess that each frame is being scaled independently instead considering a common scale factor (e.g. 65535 / (wholeStackMax - wholeStackMin )) Am I right? Do I have to program the conversion myself or something is already implemented?

Regads.

[Rasband, Wayne (NIH/NIMH) [E]]

On Mar 21, 2014, at 12:06 PM, Peter Mc wrote:

> Hi everyone;
>
> Performing a few conversions from 32-bits real images stack to 16 - bit
> images stack, I just realized that each frame is being scaled from 0 to
> 65535 so guess that each frame is being scaled independently instead
> considering a common scale factor (e.g. 65535 / (wholeStackMax -
> wholeStackMin )) Am I right? Do I have to program the conversion myself or
> something is already implemented?

The Image>Type>16-bit command converts the images in a 32-bit stack using a common scale factor of

  scale = 65535/(max-min)

were min and max define the display range that is set in the Image>Adjust>Brightness/Contrast dialog.  To convert using a scale factor based on the whole stack min and max use this simple macro

 Stack.getStatistics(voxelCount, mean, stackMin, stackMax);
 setMinAndMax(stackMin, stackMax);
 run("16-bit");

-wayne

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[Peter Mc]

Thank you Wayne.