3D Viewer problem

[Glen MacDonald-2]

Hello
The 3D Viewer plugin is creating images that are extremely magnified.  Rescaling to factors of 2 to 8 does not help.  the source volumes are only 512x512 or 640x640, 12-50 slices.  Cropping the source stack to smaller lateral dimensions only makes the result magnified even further. Nothing on the 3D Viewer website explained what might be causing this.
MacBook Pro, OS10.6.6, ImageJ 1.45b4, Java 1.6.0_24 64-bit.  

Can anyone provide an explanation?

thanks,
glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

[Benjamin Schmid-2]

Hi,

> The 3D Viewer plugin is creating images that are extremely magnified.  Rescaling to factors of 2 to 8 does not help.  the source volumes are only 512x512 or 640x640, 12-50 slices.  Cropping the source stack to smaller lateral dimensions only makes the result magnified even further. Nothing on the 3D Viewer website explained what might be causing this.
> MacBook Pro, OS10.6.6, ImageJ 1.45b4, Java 1.6.0_24 64-bit.  
>
> Can anyone provide an explanation?
>

Can you provide some information about the calibration of the stacks,
i.e. the pixel sizes as you find them in ->Image->Properties?

Best,

Bene

[Glen MacDonald-2]

Hello Bene,
Stack: 640x640x13
Properties: 0.082x0.082x 0.600 um

512x512x82
Properties: 0.077x0.077x0.10 um

Same results are obtained after cropping to smaller volumes
thanks,
Glen
On Mar 18, 2011, at 1:14 AM, Benjamin Schmid wrote:

> Hi,
>
>> The 3D Viewer plugin is creating images that are extremely magnified.  Rescaling to factors of 2 to 8 does not help.  the source volumes are only 512x512 or 640x640, 12-50 slices.  Cropping the source stack to smaller lateral dimensions only makes the result magnified even further. Nothing on the 3D Viewer website explained what might be causing this.
>> MacBook Pro, OS10.6.6, ImageJ 1.45b4, Java 1.6.0_24 64-bit.  
>>
>> Can anyone provide an explanation?
>>
>
> Can you provide some information about the calibration of the stacks,
> i.e. the pixel sizes as you find them in ->Image->Properties?
>
> Best,
>
> Bene