|
|
I have 3d images of maturing zebrafish vasculature tagged with green fluorescent protein, taken with a confocal microscope. I am running into problems with the analysis (measurement of length, area, and branching) of these images. I have been trying the skeletonize and analyzeskeleton plugins on ImageJ, but I cannot generate good 3d skeletonizations. I have been importing the image stacks as binary .tif image sequences, and I have tried setting different thresholds for the image to no avail. I would appreciate any advice.
- John
|
Locked
