3d plots for imagej

[Pedro Alejandro Herve Fernandez Carrera Licenciatura en Cs.Biologicas]

hi all, i´m trying to make a 3d surface plot, i have already use the plugin,
but i need to make the graph from a lot of pictures (coordinates),my question
is: is there a way to give imageJ X and Y coordinates, to plot them, thank´s  
a lot in advance.

Pedro A. Hervé F.
Licenciatura en Ciencias Biológicas
Universidad Austral de Chile

[Michael Ji]

Hi there,

I am a new comer.

I'd like to identify the white object within black
background for my photo.

The function of "Analysis->Analyze Particles" works
well to extact black object from white background. But
I left couple of object which is within black
background. I wonder if there is a straight way I can
do by "Analysis->Analyze Particles"

Or I can reverse my original photo, so that the black
and while is reversed. And I extract black object,
which is actually while in the original photo.

thanks,

Michael,


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[Vincenzo Della Mea]

the second possibility is the easiest one. There is a Invert item in  
the Edit menu.

Vincenzo

Il giorno 09/lug/06, alle ore 19:00, Michael Ji ha scritto:

> Hi there,
>
> I am a new comer.
>
> I'd like to identify the white object within black
> background for my photo.
>
> The function of "Analysis->Analyze Particles" works
> well to extact black object from white background. But
> I left couple of object which is within black
> background. I wonder if there is a straight way I can
> do by "Analysis->Analyze Particles"
>
> Or I can reverse my original photo, so that the black
> and while is reversed. And I extract black object,
> which is actually while in the original photo.
>
> thanks,
>
> Michael,
>
>
> __________________________________________________
> Do You Yahoo!?
> Tired of spam?  Yahoo! Mail has the best spam protection around
> http://mail.yahoo.com

* Vincenzo Della Mea
* Medical Informatics, Telemedicine and Ehealth Lab
* University od Udine, Italy
* http://mitel.dimi.uniud.it/  -  http://www.eslide.net

[Wayne Rasband]

> I'd like to identify the white object within black
> background for my photo.
>
> The function of "Analysis->Analyze Particles" works
> well to extact black object from white background. But
> I left couple of object which is within black
> background. I wonder if there is a straight way I can
> do by "Analysis->Analyze Particles"

Use the thresholding tool (Image>Adjust>Threshold) to highlight the  
white objects, then run Analyze>Analyze Particles.

-wayne

[Michael Ji]

yes, it works very well,

good product ImageJ!

Michael,

--- Vincenzo Della Mea <[hidden email]> wrote:

> the second possibility is the easiest one. There is
> a Invert item in  
> the Edit menu.
>
> Vincenzo
>
> Il giorno 09/lug/06, alle ore 19:00, Michael Ji ha
> scritto:
>
> > Hi there,
> >
> > I am a new comer.
> >
> > I'd like to identify the white object within black
> > background for my photo.
> >
> > The function of "Analysis->Analyze Particles"
> works
> > well to extact black object from white background.
> But
> > I left couple of object which is within black
> > background. I wonder if there is a straight way I
> can
> > do by "Analysis->Analyze Particles"
> >
> > Or I can reverse my original photo, so that the
> black
> > and while is reversed. And I extract black object,
> > which is actually while in the original photo.
> >
> > thanks,
> >
> > Michael,
> >
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Tired of spam?  Yahoo! Mail has the best spam
> protection around
> > http://mail.yahoo.com
>
> * Vincenzo Della Mea
> * Medical Informatics, Telemedicine and Ehealth Lab
> * University od Udine, Italy
> * http://mitel.dimi.uniud.it/  -
> http://www.eslide.net
>

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