On 7/16/2012 2:42 PM, Erika wrote:
> Hello,
>
> I am interested to know how accurate the measurements of the Particle Analyzer are for various particles, but especially nanoparticles. Any thoughts are appreciated, as well as if someone had some concrete data to share in regards to this. Perhaps there have been calibrations with standardized particles?
This is not a simple question and can not be answered by someone else
for your situation. Among the factors going into the accuracy of
measurement is the calibration of the camera that you use to take the
image in the first place. The next place that error will enter into the
analysis is the segmentation step where you try to decide which pixels
belong to a particle and which belong to background. This depends on the
nature of the particles and how well they stand out from the background
as well as any aberration related to out of focus light on various
particles. In digital images, accuracy will depend to some extent on
the ratio of particle dimension to pixel dimension.
> Please share any experiences you may have had in regards to various (nanoparticle or not) images such as accuracy when images are very clear (and only require convert-to-binary to analyze) or might be noisy (and require some manual clean up before analysis), or perhaps a bias (such as less than the correct values) when using various thresholds.
>
> Thanks,
> Erika
>
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