Activating different channels at different points in a 3-D projection?

[zwein44]

Hi everyone,

So I'm very new to imagej/ fiji and am currently attempting to make a 3-D "movie" of sorts as I rotate around two neurons. We imaged them with a confocal microscope and used three different signals: DAPI, MCM2, and Nestin. I am able to make a composite image, shave off unnecessary slices along the stack, and turn it into a 3-D projection that can rotate our view of the image by 360 degrees.

The issue I'm having however, is that I can seem to find a way to have certain channels "pop in" at different times. In short, I want to organize it so that first the few frames of the projection, we only see DAPI. At a different angle, I want to see both DAPI and MCM2, and then towards the end, I want to see all 3 channels. Is there a way to do this within the native fiji program or do I need a plugin?

Thanks!

-Zach

[Saalfeld, Stephan]

Make the complete 360deg rotation for all three variants, and then put
together parts of the resulting stacks at your liking.

http://fiji.sc/Stack-slice_Manipulations

come in handy for this.

Best,
Stephan



On Wed, 2015-07-01 at 14:07 -0700, zwein44 wrote:

> Hi everyone,
>
> So I'm very new to imagej/ fiji and am currently attempting to make a 3-D
> "movie" of sorts as I rotate around two neurons. We imaged them with a
> confocal microscope and used three different signals: DAPI, MCM2, and
> Nestin. I am able to make a composite image, shave off unnecessary slices
> along the stack, and turn it into a 3-D projection that can rotate our view
> of the image by 360 degrees.
>
> The issue I'm having however, is that I can seem to find a way to have
> certain channels "pop in" at different times. In short, I want to organize
> it so that first the few frames of the projection, we only see DAPI. At a
> different angle, I want to see both DAPI and MCM2, and then towards the end,
> I want to see all 3 channels. Is there a way to do this within the native
> fiji program or do I need a plugin?
>
> Thanks!
>
> -Zach
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Activating-different-channels-at-different-points-in-a-3-D-projection-tp5013387.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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