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Analyze particles double counting

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Analyze particles double counting

Adam Hughes
52 posts
Hi all,

I use the default analyze particles algorithm to count particles in my
picture, but noticed that if I output an image using the "Draw circles"
option, many of the circles have overlapping area.  This would mean that
the same region of my image is being double counted, leading to
overestimation of coverage in my analysis.  Does anyone know how to prevent
this behaviour?

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Re: Analyze particles double counting

Stoyan Pavlov
97 posts
Hi,
I had similar problem and I worked around it like this :
1. I ran the particle analyzer with the show masks option turned on
2. I  watershed the mask image and
Next  you have several options - you can either use the watersheded
mask image as mask for the analysis  (with the Set
Measurements=>Redirect to option, or you can add the watersheded
Regions of interest to the RoImanager using the particle analyzer.
After this you still need to examine the RoIs and see if there are any
remaining overlapping rois and delete them.
Of course this is not a perfect solution but it would work in most cases.

Stoyan
2012/9/14 Adam Hughes <[hidden email]>:

> Hi all,
>
> I use the default analyze particles algorithm to count particles in my
> picture, but noticed that if I output an image using the "Draw circles"
> option, many of the circles have overlapping area.  This would mean that
> the same region of my image is being double counted, leading to
> overestimation of coverage in my analysis.  Does anyone know how to prevent
> this behaviour?
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html



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Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
 Bulgaria
Tel: +359 (0) 52 - 677 - 086
e-mail: [hidden email]

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