"Appearance" autoscaling tones

[Jerry (Gerald) Sedgewick]

Dear List,
I’m trying to view 16-bit images so that these appear without being modified for display purposes. I would expect that setting the File>Appearance to 16-bits (versus Automatic) would then provide a view that reflects the true tonal values in the images: consistently dark images. As it is, if any image contains a bright white object the display shows a completely black background for a fluorescent image, but if an object is faint the background becomes dark gray and I can see readout noise (or whatever). I don’t want the tones in the image auto-scaled for viewing purposes. I would expect that setting the File> Appearance to 16-bits would eliminate auto-scaling of tones for display purposes.

I’m thinking it might be a problem with the Windows operating system or the graphic card? Does anyone else have this experience? I’m wanting to apply a custom LUT to these images and, as it is, the LUT applies colors variously over several images. Frustrating.

I’d very much appreciate any suggestions or feedback.

Thanks,
Jerry Sedgewick



     

           
            Jerry Sedgewick
            Imaging Consultant, Senior Management Consultant, Technical Writer
            Imaging and Analysis, LLC
            950 Cromwell Avenue
            Saint Paul, MN 55114
            651-788-2261
            [hidden email]
     

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[Gabriel Landini]

On Saturday 28 May 2016 21:49:52 Jerry  Sedgewick wrote:

> I’m trying to view 16-bit images so that these appear without being modified
> for display purposes. I would expect that setting the File>Appearance to
> 16-bits (versus Automatic) would then provide a view that reflects the true
> tonal values in the images: consistently dark images. As it is, if any
> image contains a bright white object the display shows a completely black
> background for a fluorescent image, but if an object is faint the
> background becomes dark gray and I can see readout noise (or whatever). I
> don’t want the tones in the image auto-scaled for viewing purposes. I would
> expect that setting the File> Appearance to 16-bits would eliminate
> auto-scaling of tones for display purposes.

Hi Jerry,
Currently there are no 16bit LUTs in IJ. The LUTs are all 8bits. The setting
on the Option>Appearance is for setting the range of the LUTs, but the number
of steps within that range are always 256.
Applying a range to the LUT should not modify the data in the image, only what
you see.

Cheers

Gabriel

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