There are alternative registration/alignment plugins, which allow to save
and re-apply transformation data: MultiStackReg and Image Stabilizer
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
darrenT
Sent: 19 March 2015 18:39
To:
[hidden email]
Subject: Applying one channel alignment to another (using BIJ XY offset
output?)
Hello,
I collect multipoint, multichannel data, where I regularly get XY offset due
to stage movement etc.
My DIC channel aligns wonderfully with the BIJplugin (Register ROI), which
also outputs the XY offset for each frame in a plot. My fluorescence
channels don't align at all.
So I'd like to somehow apply the XY offset data from the DIC channel to the
other channels to align all channels to the DIC.
I've already tried interleaving the 3 channels and using the first DIC frame
as the reference, but it doesn't work.
Can anyone kindly help me out with this? I'm a beginner at making macros.
Thank you very much, to an excellent community!
Darren Thomson
University of Nice Sophia-Antipolis
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