Area measurements with ImageJ

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Area measurements with ImageJ

Lena-Christin Conradi
Dear all,

maybe someone can help me to answer the following question:

I am wondering about the difference between the "freehand selection"-tool
and the  "freehand line"-tool! With both you can measure and quantify areas
of an image. The area count gives you different values, which is okay,
since you can also choose between 'pixel' and e.g. 'micrometers'.

However the problem that I encountered:
If you select an area and then another area which as approximately 50% of
the size of the first one it gives you with the "freehand selection"-tool
about 50% of the first value. However with the "freehand line"-tool I am
getting values of far more than 50%.

I would be very grateful for some explanation/advice.
Thanks,
Lena






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_______________________

Lena-Christin Conradi, MD
VIB3 - Vesalius Research Center
KULeuven Campus Gasthuisberg
O&N4 Herestraat 49
B-3000 Leuven

http://www.forschungskolleg-crc.med.uni-goettingen.de
http://www.vrc-lab.be

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Re: Area measurements with ImageJ

Jim Passmore-2
Lena,
The discrepancy isn't really a discrepancy, but the explanation is hidden
in the type of selections the two tools create.  The Freehand Selection
tool, when you draw a line, automatically connects the two endpoints and
creates an *area* selection.  The Freehand Line tool creates a *line*
selection that follows your tracing, but does not connect the endpoints.

The easiest way I can think of to illustrate this is to create an
uncalibrated image (do a Set Scale..., and click the "click to remove
scale" button if necessary), then draw a Freehand Selection and measure
Area and Length.  You'll see an area in number of "square pixels" (roughly
equal to the number of pixels enclosed by the selection), and a length of 0
(zero).  Then use the Freehand Line tool and measure the same way.  Now the
selection has a length (again, roughly the number of pixels) and it now has
an area that is very close to the length--it's the area of the selection,
length x 1 pixel wide.

The same thing applies with a length-calibrated image, but the numbers make
it more difficult to observe.  Hope this makes sense.



On Wed, May 6, 2015 at 3:04 PM, Lena-Christin Conradi <[hidden email]
> wrote:

> Dear all,
>
> maybe someone can help me to answer the following question:
>
> I am wondering about the difference between the "freehand selection"-tool
> and the  "freehand line"-tool! With both you can measure and quantify areas
> of an image. The area count gives you different values, which is okay,
> since you can also choose between 'pixel' and e.g. 'micrometers'.
>
> However the problem that I encountered:
> If you select an area and then another area which as approximately 50% of
> the size of the first one it gives you with the "freehand selection"-tool
> about 50% of the first value. However with the "freehand line"-tool I am
> getting values of far more than 50%.
>
> I would be very grateful for some explanation/advice.
> Thanks,
> Lena
>
>
>
>
>
>
> --
> _______________________
>
> Lena-Christin Conradi, MD
> VIB3 - Vesalius Research Center
> KULeuven Campus Gasthuisberg
> O&N4 Herestraat 49
> B-3000 Leuven
>
> http://www.forschungskolleg-crc.med.uni-goettingen.de
> http://www.vrc-lab.be
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>




*-- Jim Passmore*
Research Associate
Sealed Air Corporation

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html