Auto set max brightness - Color Threshold Macro

[ams376]

When manually using the Color Threshold command, ImageJ automatically sets the max brightness according to the selected image. When you put this into a macro using the recording tool it will set the max brightness to a fixed value. Is there a way to create a Color Threshold macro that doesn't use a fixed max, but automatically sets it according to the selected image like how the command works when using it manually.? Macro posted below.. The line I'm referring to - "max[2]=10;".



//run("Threshold...");
// Color Thresholder 1.48v
// Autogenerated macro, single images only!
min=newArray(3);
max=newArray(3);
filter=newArray(3);
a=getTitle();
run("HSB Stack");
run("Convert Stack to Images");
selectWindow("Hue");
rename("0");
selectWindow("Saturation");
rename("1");
selectWindow("Brightness");
rename("2");
min[0]=0;
max[0]=255;
filter[0]="pass";
min[1]=0;
max[1]=255;
filter[1]="pass";
min[2]=0;
max[2]=10;
filter[2]="pass";
for (i=0;i<3;i++){
  selectWindow(""+i);
  setThreshold(min[i], max[i]);
  run("Convert to Mask");
  if (filter[i]=="stop")  run("Invert");
}
imageCalculator("AND create", "0","1");
imageCalculator("AND create", "Result of 0","2");
for (i=0;i<3;i++){
  selectWindow(""+i);
  close();
}
selectWindow("Result of 0");
close();
selectWindow("Result of Result of 0");
rename(a);
// Colour Thresholding-------------
setOption("BlackBackground", false);

[Ryan Smith]

I also need a solution for this problem: auto setting max brightness value in the color threshold macro. Please help community!

[shubham1278]

Hi,

I found the answer to this issue. You can change the macro as follows

//run("Threshold...");
// Color Thresholder 1.48v
// Autogenerated macro, single images only!
min=newArray(3);
max=newArray(3);
filter=newArray(3);
a=getTitle();
run("HSB Stack");
run("Convert Stack to Images");
selectWindow("Hue");
rename("0");
selectWindow("Saturation");
rename("1");
selectWindow("Brightness");
rename("2");
selectWindow("2");
setAutoThreshold("Default");
//run("Threshold...");
getThreshold(lower,upper);
resetThreshold();
//run("Close");

//print(lower+","+upper);

min[0]=0;
max[0]=255;
filter[0]="pass";
min[1]=0;
max[1]=255;
filter[1]="pass";
min[2]=lower;
max[2]=upper;
filter[2]="pass";
for (i=0;i<3;i++){
  selectWindow(""+i);
  setThreshold(min[i], max[i]);
  run("Convert to Mask");
  if (filter[i]=="stop")  run("Invert");
}
imageCalculator("AND create", "0","1");
imageCalculator("AND create", "Result of 0","2");
for (i=0;i<3;i++){
  selectWindow(""+i);
  close();
}
selectWindow("Result of 0");
close();
selectWindow("Result of Result of 0");
rename(a);
// Colour Thresholding-------------
setOption("BlackBackground", false);

Best,
Shubham



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