Autofluorescence elimination

[Tomás Gutiérrez]

Hi everyone,

I am trying to identify a particular cell type in a paraffin embebed tissue sample via immunohistochemistry, but I found that a large number of cells show autofluorescence. I suppose I could capture the autofluorescence in an unspecific wavelength and do a subtraction, but I don't think this is really correct.

Is there an "imagej way" to solve the autofluorescence problem or I should just try a chemical pretreatment of the sample?

Thank you.


Tomás Gutiérrez, MSc
Universidad de Chile