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I stitched 9 images (of the insular cortex in a rat brain) together taken at 10x. However I can't seem to automate my cell counting on the stitched image. For instance, the normal protocol states to switch the image over to 8bit, but the stitched/fused image is already in 8-bit format (yet still with color). In addition when I try to fiddle around with the threshold, it essentially does nothing.
Has anyone else had an issue with this? Or know of better ways to automate counting cells for a region that isn't captured by the field of the microscope?
Thank you.
-Brian
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