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I am trying to track a single tadpole as it swims across a Petri dish using the Object Tracker plugin by Wayne Rasband. I tried to follow the directions for a test stack from Ross Lang but there was no mention of how exactly to select which two objects I want to be tracked, except when removing the objects from the background image. Is this the only selection I need and what should I do since I only need to track one object? Also, there is some movement of a pipette in the video (but it never blocks the tadpole) that I am concerned may be affecting the programs ability to track the tadpole. The pipette and ripples in the water become red after I adjust the threshold and the directions for the test stack say to make sure nothing is red except the objects to be tracked. Is this a problem and is there a way around this? I'd greatly appreciate any help or information on another program or plugin that may be better suited for this type of automatic tracking. Thank you.
George
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