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I was hoping someone would be able to help me out with PunctaAnalyzer. I think the software is great for my purpose of quantifying the number of colocalized synapses in a given image. However, I have found two major drawbacks. (1) PA is only compatiable with ImageJ 1.29 or under and (2) you cannot automate/create a macro for the plugin
My question comes from one of these drawbacks. I have about 800 images I need to analyze with punctaanalyzer. Previously I did about 300 manually but it took me hours and hours and was a huge pain. I was wondering if anyone knew of a way to automate this process. Specifically, I want to use the same exact threshhold, rolling ball radius, results output, etc.
I would really appreciate help with this, if it's possible. thanks!
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