Dear Adrian,
You can use Process > Image Calculator to subtract images. There is also the option to use Process > Subtract Background... what uses a rolling background approach to correct uneven illuminated background, see:
https://imagej.nih.gov/ij/docs/guide/146-29.html#toc-Subsection-29.14Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif-----Original Message-----
From: Adrián Villalba [mailto:
[hidden email]]
Sent: 07 June 2017 09:35
To:
[hidden email]
Subject: Background substracting except for a ROI
Dear all,
I use to substract background in fluorescence images (1 channel) by substracting the mean value setted by measurements. In the case that the background is not uniform i would like to know if it possible to substract the entire image without the ROI from the original image.
Thank you all in advance,
--
- Adrián Villalba Felipe.
https://es.linkedin.com/in/adrianvillalba--
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