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I'm trying to background subtract stacks of 16-bit images for which there is no dark-frame. The images are like the "m51" sample where you have a large central spot and a background close to 0. Thus, rolling ball background subtraction won't work because it always takes off signal instead of noise. I need to a true background that averages to 0 (both positive and negative values) or the power calibration in the image will be off. Because the stacks have many frames and the spot moves around, I need to do the subtraction in an automated fashion on a frame-by-frame basis. Anyone done something similar?
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