Dear All,
I am trying to analyze mitochondrial content using images obtained through immunofluorescence. As well as the mitochondria image, I also have a cell border image.
Using ImageJ, is it firstly possible to analyze mitochondrial content in each individual cell (approximately 12 cells per image).
Secondly, I am interested in analyzing mitochondrial content close to the plasma membrane along a continuum towards the centre of the cell. Therefore, I would hope to gain data showing greater mitochondrial content in subsarcolemmal regions compared to the middle of the cell. Is there a plugin which would enable me to achieve this?
Any help would be much appreciated.
Cheers,
Sam
Sam Shepherd
PhD Student
Human Performance Laboratory
Exercise Metabolism Research Group
School of Sport & Exercise Sciences
The University of Birmingham
Edgbaston,
Birmingham,
West Midlands,
B15 2TT
T: 0121 414 8746
M: 07824 638296
E:
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