Defne,
please specify what you mean by "reliable".
>Hi All,
>
>How reliable is quantification with ImageJ for an image that is
>converted to a TIFF, which had intially started out as a JPEG?
You won't loose any information by converting a JPEG-image to TIFF,
but if you are interested in scientific image evaluation, the use of
JPEG-images in the processing chain is definitely a no-no.
Although lossless JPEG-compression exists, JPEG generally means a
considerable loss of information and the introduction of artifacts.
>Also, although it is a recurring question, I would like to inquire
>on why certain
>TIFF images I have (of ethidium and coomassie stains) seem to
>randomly require a
>negative of the image/bands for correct quantification. Does this make the
>quantification less reliable, or is it just a matter of trusting the
>absolute values
>(the net difference from the background band)?
This question is up to those who are experineced in gel evaluation.
>Thank you very much,
>
>Defne
Best
--
Herbie
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