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Hi, for my research work I have to simultaneously adjust the brightness/contrast for all the pictures in a stack to separate the a shrinking droplet from background. All versions of ImageJ later than 1.40f that I've attempted can only adjust the current image. Even with the dialogue window asking me"apply settings to all slices in the stack?" I clicked "Ok," still only the current slices has been adjusted.
I searched the forum before I posted this mail, and found that someone came across the similar problem to me. The answer said that it was because the stack is a composite stack. In my case, I am kind of not sure whether my stack is composite or not and I've also tried to convert the stack to other formats, but the problem persists.
Use "image"->"show info", I get the following information:
[quote]
Title: 24sep2009_casein_70_1mcl_repea2
Width: 720 pixels
Height: 576 pixels
Depth: 50 pixels
ID: -159
Coordinate origin: 0,0,0
Bits per pixel: 32 (RGB)
Image: 27/50 (24sep2009_casein_70_1mcl_repea2_00026)
No Threshold
Uncalibrated
Path: G:\E\video\20090924\24sep2009_casein_70_1mcl_repea2\
No Selection
[/quote]
The problem happens to two computers, one with a 32bit Win Xp, and another with a 64bit Win7 (with 64bit Java). On the 32bit Xp PC, if I degraded the software to 1.40f, the problem can be solved, but for the 64bit Win7, which is my current working PC, I have no idea what I should do so that I can do the batch adjustment of the brightness/contrast for all the slices in a stack.
I highly appreciate any help to my question! Thanks!
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