Batch split channels and save pictures from YFP-CFP FRET

[nan2ying]

Hello guys,

I saw a earlier question about "batch split channels and save", http://imagej.588099.n2.nabble.com/Batch-split-channels-and-save-td5377696.html#none. But this doesn't work on my pictures taken from YFP-CFP FRET experiments. The difference is that my picture is not a regular RGB 24-bit picture, whereas it's a LSM 16-bit file.

I wonder if someone could help me edit the previous macro to fit my purpose. I guess it should not be very difficult for experts, while I'm just a beginner using imageJ. Thanks a lot.

BTW, I don't know how to open the command window. Could someone tell me about this?

Nan

[nan2ying]

What I've done is change the "bitDepth!=24" to "bitDepth!=16", and delete
"saveAs("Tiff", dir2+title+"d2.tif");
 close();"
because I only have two channels.

This is all I could figure out. But it turned out that my picture was "successfully split", but their is nothing in the split pictures.

Nan