Surya,
Any chance you can attach an image showing what you're looking at and
what you're trying to count and assign to particular cells? I can't
tell from your description, for example, whether the cells are somewhat
translucent and you are looking at a culture more than one cell thick
and trying to match the dots in 3D, not just 2D.
Wade
On 7/22/12 3:06 PM, Surya Reis wrote:
> Hello,
>
> which is the best method to identify individual cells in dense
> cultures? I am trying to count perinuclear/ cytoplasmic dots per cell
> in dense cultures.
>
> thank you,
>
> Surya
>
>
>
>
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