Calculate area on a per object basis

[danih]

Dear List,

I have images with two channels (red and green).  I'd like to first segment the red objects and then calculate the amount (area) of green signal per red object, on an object by object basis.

I can easily calculate the area of green contained within the red with the script below, but I can't figure out how to get the area of green per red object.  Is it possible?  Any and all advice is very welcome!!

Thanks tons!

Danielle

p.s. I hope to automate/batch this analysis.

imgName=getTitle();
run("Split Channels");
selectWindow(imgName + " (blue)");
close();
selectWindow(imgName + " (red)");
setAutoThreshold("Default");
//run("Threshold...");
setThreshold(40, 255);
setOption("BlackBackground", false);
run("Convert to Mask");
run("Analyze Particles...", "size=50-Infinity show=Outlines summarize");
selectWindow(imgName + " (red)");
selectWindow("Drawing of " + imgName + " (red)");
close();
selectWindow(imgName + " (red)");
//run("Threshold...");
setThreshold(0, 243);
run("Convert to Mask");
imageCalculator("Subtract create", imgName + " (green)", imgName + " (red)");
selectWindow("Result of " + imgName + " (green)");
setAutoThreshold("Default");
//run("Threshold...");
setThreshold(75, 255);
run("Convert to Mask");
run("Analyze Particles...", "size=0-Infinity show=Outlines summarize");



This message is intended only for the confidential use of the intended recipient(s). If you have received this communication in error, please notify the sender by reply e-mail, and delete the original message and any attachments. Any unauthorized disclosure, copying, or distribution of this message (including the attachments), or the taking of any action based on it, is strictly prohibited.

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[Avital Steinberg]

Hi Danielle,
If I understand you correctly, you have large red objects which may contain more than one green objects within them. If this is the case, after you segment the red objects, you can use them as an overlay on the green channel.

Then, you can iterate over the red objects, use adjust, threshold for each ROI separately, and then you'll be able to keep track: which green objects are within which red object.

I hope I understood your problem correctly.

Good luck,
Avital

Sent from my iPhone

> On Sep 9, 2015, at 6:35 AM, Danielle Harmon <[hidden email]> wrote:
>
> Dear List,
>
> I have images with two channels (red and green).  I'd like to first segment the red objects and then calculate the amount (area) of green signal per red object, on an object by object basis.
>
> I can easily calculate the area of green contained within the red with the script below, but I can't figure out how to get the area of green per red object.  Is it possible?  Any and all advice is very welcome!!
>
> Thanks tons!
>
> Danielle
>
> p.s. I hope to automate/batch this analysis.
>
> imgName=getTitle();
> run("Split Channels");
> selectWindow(imgName + " (blue)");
> close();
> selectWindow(imgName + " (red)");
> setAutoThreshold("Default");
> //run("Threshold...");
> setThreshold(40, 255);
> setOption("BlackBackground", false);
> run("Convert to Mask");
> run("Analyze Particles...", "size=50-Infinity show=Outlines summarize");
> selectWindow(imgName + " (red)");
> selectWindow("Drawing of " + imgName + " (red)");
> close();
> selectWindow(imgName + " (red)");
> //run("Threshold...");
> setThreshold(0, 243);
> run("Convert to Mask");
> imageCalculator("Subtract create", imgName + " (green)", imgName + " (red)");
> selectWindow("Result of " + imgName + " (green)");
> setAutoThreshold("Default");
> //run("Threshold...");
> setThreshold(75, 255);
> run("Convert to Mask");
> run("Analyze Particles...", "size=0-Infinity show=Outlines summarize");
>
>
>
> This message is intended only for the confidential use of the intended recipient(s). If you have received this communication in error, please notify the sender by reply e-mail, and delete the original message and any attachments. Any unauthorized disclosure, copying, or distribution of this message (including the attachments), or the taking of any action based on it, is strictly prohibited.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Straub, Volko A. (Dr.)]

Hi Danielle,

Further to Avital suggestion, you could also add all the regions
selected by the last line of your code to the ROI manager and then use a
For-loop to run through all the regions to carry out individual
measurements on each ROI.
Something like:
run("Analyze Particles...", "size=0-Infinity show=Outlines summarize add");
for(i=1;i<roiManager("count")+1;i++){
     roiManager("Select",i);
     run("Measure");
}

Hope this helps,
Volko

On 09/09/2015 06:01, Avital Steinberg wrote:

> Hi Danielle,
> If I understand you correctly, you have large red objects which may contain more than one green objects within them. If this is the case, after you segment the red objects, you can use them as an overlay on the green channel.
>
> Then, you can iterate over the red objects, use adjust, threshold for each ROI separately, and then you'll be able to keep track: which green objects are within which red object.
>
> I hope I understood your problem correctly.
>
> Good luck,
> Avital
>
> Sent from my iPhone
>
>> On Sep 9, 2015, at 6:35 AM, Danielle Harmon <[hidden email]> wrote:
>>
>> Dear List,
>>
>> I have images with two channels (red and green).  I'd like to first segment the red objects and then calculate the amount (area) of green signal per red object, on an object by object basis.
>>
>> I can easily calculate the area of green contained within the red with the script below, but I can't figure out how to get the area of green per red object.  Is it possible?  Any and all advice is very welcome!!
>>
>> Thanks tons!
>>
>> Danielle
>>
>> p.s. I hope to automate/batch this analysis.
>>
>> imgName=getTitle();
>> run("Split Channels");
>> selectWindow(imgName + " (blue)");
>> close();
>> selectWindow(imgName + " (red)");
>> setAutoThreshold("Default");
>> //run("Threshold...");
>> setThreshold(40, 255);
>> setOption("BlackBackground", false);
>> run("Convert to Mask");
>> run("Analyze Particles...", "size=50-Infinity show=Outlines summarize");
>> selectWindow(imgName + " (red)");
>> selectWindow("Drawing of " + imgName + " (red)");
>> close();
>> selectWindow(imgName + " (red)");
>> //run("Threshold...");
>> setThreshold(0, 243);
>> run("Convert to Mask");
>> imageCalculator("Subtract create", imgName + " (green)", imgName + " (red)");
>> selectWindow("Result of " + imgName + " (green)");
>> setAutoThreshold("Default");
>> //run("Threshold...");
>> setThreshold(75, 255);
>> run("Convert to Mask");
>> run("Analyze Particles...", "size=0-Infinity show=Outlines summarize");
>>
>>
>>
>> This message is intended only for the confidential use of the intended recipient(s). If you have received this communication in error, please notify the sender by reply e-mail, and delete the original message and any attachments. Any unauthorized disclosure, copying, or distribution of this message (including the attachments), or the taking of any action based on it, is strictly prohibited.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Avital Steinberg]

Yes - this is exactly what I meant: iterate over the red objects, set the
threshold and segment the green particles within the red particles.

Good luck,
Avital

On Wed, Sep 9, 2015 at 8:35 AM, Volko Straub <[hidden email]> wrote:

> Hi Danielle,
>
> Further to Avital suggestion, you could also add all the regions selected
> by the last line of your code to the ROI manager and then use a For-loop to
> run through all the regions to carry out individual measurements on each
> ROI.
> Something like:
> run("Analyze Particles...", "size=0-Infinity show=Outlines summarize add");
> for(i=1;i<roiManager("count")+1;i++){
>     roiManager("Select",i);
>     run("Measure");
> }
>
> Hope this helps,
> Volko
>
>
> On 09/09/2015 06:01, Avital Steinberg wrote:
>
>> Hi Danielle,
>> If I understand you correctly, you have large red objects which may
>> contain more than one green objects within them. If this is the case, after
>> you segment the red objects, you can use them as an overlay on the green
>> channel.
>>
>> Then, you can iterate over the red objects, use adjust, threshold for
>> each ROI separately, and then you'll be able to keep track: which green
>> objects are within which red object.
>>
>> I hope I understood your problem correctly.
>>
>> Good luck,
>> Avital
>>
>> Sent from my iPhone
>>
>> On Sep 9, 2015, at 6:35 AM, Danielle Harmon <[hidden email]> wrote:
>>>
>>> Dear List,
>>>
>>> I have images with two channels (red and green).  I'd like to first
>>> segment the red objects and then calculate the amount (area) of green
>>> signal per red object, on an object by object basis.
>>>
>>> I can easily calculate the area of green contained within the red with
>>> the script below, but I can't figure out how to get the area of green per
>>> red object.  Is it possible?  Any and all advice is very welcome!!
>>>
>>> Thanks tons!
>>>
>>> Danielle
>>>
>>> p.s. I hope to automate/batch this analysis.
>>>
>>> imgName=getTitle();
>>> run("Split Channels");
>>> selectWindow(imgName + " (blue)");
>>> close();
>>> selectWindow(imgName + " (red)");
>>> setAutoThreshold("Default");
>>> //run("Threshold...");
>>> setThreshold(40, 255);
>>> setOption("BlackBackground", false);
>>> run("Convert to Mask");
>>> run("Analyze Particles...", "size=50-Infinity show=Outlines summarize");
>>> selectWindow(imgName + " (red)");
>>> selectWindow("Drawing of " + imgName + " (red)");
>>> close();
>>> selectWindow(imgName + " (red)");
>>> //run("Threshold...");
>>> setThreshold(0, 243);
>>> run("Convert to Mask");
>>> imageCalculator("Subtract create", imgName + " (green)", imgName + "
>>> (red)");
>>> selectWindow("Result of " + imgName + " (green)");
>>> setAutoThreshold("Default");
>>> //run("Threshold...");
>>> setThreshold(75, 255);
>>> run("Convert to Mask");
>>> run("Analyze Particles...", "size=0-Infinity show=Outlines summarize");
>>>
>>>
>>>
>>> This message is intended only for the confidential use of the intended
>>> recipient(s). If you have received this communication in error, please
>>> notify the sender by reply e-mail, and delete the original message and any
>>> attachments. Any unauthorized disclosure, copying, or distribution of this
>>> message (including the attachments), or the taking of any action based on
>>> it, is strictly prohibited.
>>>
>>> --
>>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[jerie]

Danielle,

you may want to look into

http://imagej.nih.gov/ij/docs/menus/analyze.html#set
"...
Redirect To - The image selected from this popup menu will be used as the target for statistical calculations done by the Measure and Analyze Particles commands. The Redirect To feature allows you to outline a structure on one image and measure the intensity of the corresponding region in another image. With ImageJ 1.35d or later this feature also works with stacks. Note that it is the thresholding of the target image that is used when Limit to Threshold is enabled.
..."
So, threshold both images, then Analyse particles in the red channel but redirect the measurement to the green channel with 'Limit to Threshold' checked will return the selected measurements, like area (and vice versa.).

Good success, Jens

[Kyaw Moe]

Dear All
                     I am Kyaw Moe, studying Dentistry in South Korea for
PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji to
measure the remaining untouched surface area of root canal after shaping
with NiTi Rotary files. As a beginner, although I read this article, I am
not so clear and cannot  follow their steps.  I am interested in ImageJ
software to apply for my experiment. I would be very happy if you give the
detail instruction for that procedure. Please help me.
Looking forward to seeing your help.

With Regards
Sincerely
Kyaw Moe

On Thu, Sep 10, 2015 at 12:15 PM, Kyaw Moe <[hidden email]> wrote:

> Dear All
>                      I am Kyaw Moe, studying Dentistry in South Korea for
> PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji to
> measure the remaining untouched surface area of root canal after shaping
> with NiTi Rotary files. As a beginner, although I read this article, I am
> not so clear and cannot  follow their steps.  I am interested in ImageJ
> software to apply for my experiment. I would be very happy if you give the
> detail instruction for that procedure. I attached the article which used
> ImageJ. Please help me.
> Looking forward to seeing your help.
>
> With Regards
> Sincerely
> Kyaw Moe
>
> On Thu, Sep 10, 2015 at 5:54 AM, jerie <[hidden email]> wrote:
>
>> Danielle,
>>
>> you may want to look into
>>
>> http://imagej.nih.gov/ij/docs/menus/analyze.html#set
>> "...
>> Redirect To - The image selected from this popup menu will be used as the
>> target for statistical calculations done by the Measure and Analyze
>> Particles commands. The Redirect To feature allows you to outline a
>> structure on one image and measure the intensity of the corresponding
>> region
>> in another image. With ImageJ 1.35d or later this feature also works with
>> stacks. Note that it is the thresholding of the target image that is used
>> when Limit to Threshold is enabled.
>> ..."
>> So, threshold both images, then Analyse particles in the red channel but
>> redirect the measurement to the green channel with 'Limit to Threshold'
>> checked will return the selected measurements, like area (and vice
>> versa.).
>>
>> Good success, Jens
>>
>>
>>
>>
>>
>> -----
>> Jens Rietdorf
>> Visiting Scientist
>> Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento
>> Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.
>> --
>> View this message in context:
>> http://imagej.1557.x6.nabble.com/Calculate-area-on-a-per-object-basis-tp5014278p5014298.html
>> Sent from the ImageJ mailing list archive at Nabble.com.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Avital Steinberg]

Hi Kyaw,
It's a good idea to write a separate email (rather than to reply to an
email, like you did now) to the ImageJ list, because this is a thread
discussing Danielle's question. (and the people who are following it are
only the ones who thought they may be able to help Danielle) Also, it's a
good idea to attach the article that you're referring to in your question.

Good luck,
Avital

On Thu, Sep 10, 2015 at 6:21 AM, Kyaw Moe <[hidden email]> wrote:

> Dear All
>                      I am Kyaw Moe, studying Dentistry in South Korea for
> PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji to
> measure the remaining untouched surface area of root canal after shaping
> with NiTi Rotary files. As a beginner, although I read this article, I am
> not so clear and cannot  follow their steps.  I am interested in ImageJ
> software to apply for my experiment. I would be very happy if you give the
> detail instruction for that procedure. Please help me.
> Looking forward to seeing your help.
>
> With Regards
> Sincerely
> Kyaw Moe
>
> On Thu, Sep 10, 2015 at 12:15 PM, Kyaw Moe <[hidden email]> wrote:
>
> > Dear All
> >                      I am Kyaw Moe, studying Dentistry in South Korea for
> > PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji
> to
> > measure the remaining untouched surface area of root canal after shaping
> > with NiTi Rotary files. As a beginner, although I read this article, I am
> > not so clear and cannot  follow their steps.  I am interested in ImageJ
> > software to apply for my experiment. I would be very happy if you give
> the
> > detail instruction for that procedure. I attached the article which used
> > ImageJ. Please help me.
> > Looking forward to seeing your help.
> >
> > With Regards
> > Sincerely
> > Kyaw Moe
> >
> > On Thu, Sep 10, 2015 at 5:54 AM, jerie <[hidden email]> wrote:
> >
> >> Danielle,
> >>
> >> you may want to look into
> >>
> >> http://imagej.nih.gov/ij/docs/menus/analyze.html#set
> >> "...
> >> Redirect To - The image selected from this popup menu will be used as
> the
> >> target for statistical calculations done by the Measure and Analyze
> >> Particles commands. The Redirect To feature allows you to outline a
> >> structure on one image and measure the intensity of the corresponding
> >> region
> >> in another image. With ImageJ 1.35d or later this feature also works
> with
> >> stacks. Note that it is the thresholding of the target image that is
> used
> >> when Limit to Threshold is enabled.
> >> ..."
> >> So, threshold both images, then Analyse particles in the red channel but
> >> redirect the measurement to the green channel with 'Limit to Threshold'
> >> checked will return the selected measurements, like area (and vice
> >> versa.).
> >>
> >> Good success, Jens
> >>
> >>
> >>
> >>
> >>
> >> -----
> >> Jens Rietdorf
> >> Visiting Scientist
> >> Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento
> >> Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.
> >> --
> >> View this message in context:
> >>
> http://imagej.1557.x6.nabble.com/Calculate-area-on-a-per-object-basis-tp5014278p5014298.html
> >> Sent from the ImageJ mailing list archive at Nabble.com.
> >>
> >> --
> >> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >>
> >
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
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[Kyaw Moe]

Hi Avital
               Sorry for that. I saw you all are discussing about surface
area. So. I thought someone in this group may help me. Actually, I don't
know who can help me. If you know, please give me their email or the way
where I can get.

              Sorry again for my mistake.
Regards
Kyaw Moe

On Thu, Sep 10, 2015 at 2:10 PM, Avital Steinberg <[hidden email]
> wrote:

> Hi Kyaw,
> It's a good idea to write a separate email (rather than to reply to an
> email, like you did now) to the ImageJ list, because this is a thread
> discussing Danielle's question. (and the people who are following it are
> only the ones who thought they may be able to help Danielle) Also, it's a
> good idea to attach the article that you're referring to in your question.
>
> Good luck,
> Avital
>
> On Thu, Sep 10, 2015 at 6:21 AM, Kyaw Moe <[hidden email]> wrote:
>
> > Dear All
> >                      I am Kyaw Moe, studying Dentistry in South Korea for
> > PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji
> to
> > measure the remaining untouched surface area of root canal after shaping
> > with NiTi Rotary files. As a beginner, although I read this article, I am
> > not so clear and cannot  follow their steps.  I am interested in ImageJ
> > software to apply for my experiment. I would be very happy if you give
> the
> > detail instruction for that procedure. Please help me.
> > Looking forward to seeing your help.
> >
> > With Regards
> > Sincerely
> > Kyaw Moe
> >
> > On Thu, Sep 10, 2015 at 12:15 PM, Kyaw Moe <[hidden email]> wrote:
> >
> > > Dear All
> > >                      I am Kyaw Moe, studying Dentistry in South Korea
> for
> > > PhD in Conservative Dentistry course. I saw one article used ImageJ
> Fiji
> > to
> > > measure the remaining untouched surface area of root canal after
> shaping
> > > with NiTi Rotary files. As a beginner, although I read this article, I
> am
> > > not so clear and cannot  follow their steps.  I am interested in ImageJ
> > > software to apply for my experiment. I would be very happy if you give
> > the
> > > detail instruction for that procedure. I attached the article which
> used
> > > ImageJ. Please help me.
> > > Looking forward to seeing your help.
> > >
> > > With Regards
> > > Sincerely
> > > Kyaw Moe
> > >
> > > On Thu, Sep 10, 2015 at 5:54 AM, jerie <[hidden email]> wrote:
> > >
> > >> Danielle,
> > >>
> > >> you may want to look into
> > >>
> > >> http://imagej.nih.gov/ij/docs/menus/analyze.html#set
> > >> "...
> > >> Redirect To - The image selected from this popup menu will be used as
> > the
> > >> target for statistical calculations done by the Measure and Analyze
> > >> Particles commands. The Redirect To feature allows you to outline a
> > >> structure on one image and measure the intensity of the corresponding
> > >> region
> > >> in another image. With ImageJ 1.35d or later this feature also works
> > with
> > >> stacks. Note that it is the thresholding of the target image that is
> > used
> > >> when Limit to Threshold is enabled.
> > >> ..."
> > >> So, threshold both images, then Analyse particles in the red channel
> but
> > >> redirect the measurement to the green channel with 'Limit to
> Threshold'
> > >> checked will return the selected measurements, like area (and vice
> > >> versa.).
> > >>
> > >> Good success, Jens
> > >>
> > >>
> > >>
> > >>
> > >>
> > >> -----
> > >> Jens Rietdorf
> > >> Visiting Scientist
> > >> Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento
> > >> Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.
> > >> --
> > >> View this message in context:
> > >>
> >
> http://imagej.1557.x6.nabble.com/Calculate-area-on-a-per-object-basis-tp5014278p5014298.html
> > >> Sent from the ImageJ mailing list archive at Nabble.com.
> > >>
> > >> --
> > >> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> > >>
> > >
> > >
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
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[BenTupper]

Hi Kyaw Moe,

You might get more detailed responses if you...

1. post a small example of an image to a public repository or to a public dropbox and shared the URL to that file in a new email thread,

2. show what steps you have tried so far.

Best wishes,
Ben

On Sep 10, 2015, at 2:22 AM, Kyaw Moe <[hidden email]> wrote:

> Hi Avital
>               Sorry for that. I saw you all are discussing about surface
> area. So. I thought someone in this group may help me. Actually, I don't
> know who can help me. If you know, please give me their email or the way
> where I can get.
>
>              Sorry again for my mistake.
> Regards
> Kyaw Moe
>
> On Thu, Sep 10, 2015 at 2:10 PM, Avital Steinberg <[hidden email]
>> wrote:
>
>> Hi Kyaw,
>> It's a good idea to write a separate email (rather than to reply to an
>> email, like you did now) to the ImageJ list, because this is a thread
>> discussing Danielle's question. (and the people who are following it are
>> only the ones who thought they may be able to help Danielle) Also, it's a
>> good idea to attach the article that you're referring to in your question.
>>
>> Good luck,
>> Avital
>>
>> On Thu, Sep 10, 2015 at 6:21 AM, Kyaw Moe <[hidden email]> wrote:
>>
>>> Dear All
>>>                     I am Kyaw Moe, studying Dentistry in South Korea for
>>> PhD in Conservative Dentistry course. I saw one article used ImageJ Fiji
>> to
>>> measure the remaining untouched surface area of root canal after shaping
>>> with NiTi Rotary files. As a beginner, although I read this article, I am
>>> not so clear and cannot  follow their steps.  I am interested in ImageJ
>>> software to apply for my experiment. I would be very happy if you give
>> the
>>> detail instruction for that procedure. Please help me.
>>> Looking forward to seeing your help.
>>>
>>> With Regards
>>> Sincerely
>>> Kyaw Moe
>>>
>>> On Thu, Sep 10, 2015 at 12:15 PM, Kyaw Moe <[hidden email]> wrote:
>>>
>>>> Dear All
>>>>                     I am Kyaw Moe, studying Dentistry in South Korea
>> for
>>>> PhD in Conservative Dentistry course. I saw one article used ImageJ
>> Fiji
>>> to
>>>> measure the remaining untouched surface area of root canal after
>> shaping
>>>> with NiTi Rotary files. As a beginner, although I read this article, I
>> am
>>>> not so clear and cannot  follow their steps.  I am interested in ImageJ
>>>> software to apply for my experiment. I would be very happy if you give
>>> the
>>>> detail instruction for that procedure. I attached the article which
>> used
>>>> ImageJ. Please help me.
>>>> Looking forward to seeing your help.
>>>>
>>>> With Regards
>>>> Sincerely
>>>> Kyaw Moe
>>>>
>>>> On Thu, Sep 10, 2015 at 5:54 AM, jerie <[hidden email]> wrote:
>>>>
>>>>> Danielle,
>>>>>
>>>>> you may want to look into
>>>>>
>>>>> http://imagej.nih.gov/ij/docs/menus/analyze.html#set
>>>>> "...
>>>>> Redirect To - The image selected from this popup menu will be used as
>>> the
>>>>> target for statistical calculations done by the Measure and Analyze
>>>>> Particles commands. The Redirect To feature allows you to outline a
>>>>> structure on one image and measure the intensity of the corresponding
>>>>> region
>>>>> in another image. With ImageJ 1.35d or later this feature also works
>>> with
>>>>> stacks. Note that it is the thresholding of the target image that is
>>> used
>>>>> when Limit to Threshold is enabled.
>>>>> ..."
>>>>> So, threshold both images, then Analyse particles in the red channel
>> but
>>>>> redirect the measurement to the green channel with 'Limit to
>> Threshold'
>>>>> checked will return the selected measurements, like area (and vice
>>>>> versa.).
>>>>>
>>>>> Good success, Jens
>>>>>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>> -----
>>>>> Jens Rietdorf
>>>>> Visiting Scientist
>>>>> Fundação Oswaldo Cruz - Ministério da Saúde, Centro de Desenvolvimento
>>>>> Tecnológico em Saúde (CDTS), Rio de Janeiro, Brasil.
>>>>> --
>>>>> View this message in context:
>>>>>
>>>
>> http://imagej.1557.x6.nabble.com/Calculate-area-on-a-per-object-basis-tp5014278p5014298.html
>>>>> Sent from the ImageJ mailing list archive at Nabble.com.
>>>>>
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>>>>>
>>>>
>>>>
>>>
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>>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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>>
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>
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