Can I invert Grey Units?

Previous Topic Next Topic
 
classic Classic list List threaded Threaded
2 messages Options
Reply | Threaded
Open this post in threaded view
|

Can I invert Grey Units?

Athomp40
My image has significantly more relevant data the darker the grey tone. When i use imagej and analyze my data using "Measure" it attributes the whitest color as the highest grey unit of 255 and black is 0 grey units. I guess my question is can I change the grey scale settings (or calibrate) so that as the higher grey units increase it darkens closer to black (attributing the blackest a value of 255)?  
Reply | Threaded
Open this post in threaded view
|

Re: Can I invert Grey Units?

Rasband, Wayne (NIH/NIMH) [E]
On Apr 16, 2014, at 9:12 PM, Athomp40 wrote:

> My image has significantly more relevant data the darker the grey tone. When
> i use imagej and analyze my data using "Measure" it attributes the whitest
> color as the highest grey unit of 255 and black is 0 grey units. I guess my
> question is can I change the grey scale settings (or calibrate) so that as
> the higher grey units increase it darkens closer to black (attributing the
> blackest a value of 255)?

Invert the image (Edit>Invert) and invert the LUT (Image>Lookup Tables>Invert LUT), or use the Analyze>Calibrate command and select "Pixel Inverter" in the dialog as the calibration function.

-wayne

> --
> View this message in context: http://imagej.1557.x6.nabble.com/Can-I-invert-Grey-Units-tp5007330.html
> Sent from the ImageJ mailing list archive at Nabble.com.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html