On Apr 16, 2014, at 9:12 PM, Athomp40 wrote:
> My image has significantly more relevant data the darker the grey tone. When
> i use imagej and analyze my data using "Measure" it attributes the whitest
> color as the highest grey unit of 255 and black is 0 grey units. I guess my
> question is can I change the grey scale settings (or calibrate) so that as
> the higher grey units increase it darkens closer to black (attributing the
> blackest a value of 255)?
Invert the image (Edit>Invert) and invert the LUT (Image>Lookup Tables>Invert LUT), or use the Analyze>Calibrate command and select "Pixel Inverter" in the dialog as the calibration function.
-wayne
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