Cell counter issue

[Flemming Ravn Merkel]

Hi All

I use the Cell Counter plugin to mark and count seabirds on large composite images. Cell Counter automatically sum up all the different marker types for the entire image. However, occasionally I need to to sum up the markers for only a subarea of the image or to split up the total count according a grid overlay.

Any idea how to do this?

Kindly
Flemming

 
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[Cammer, Michael]

Cell counter marks each criteria with different color.

Perhaps you could flatten the image thus stamping in the overlays, then draw ROI, segment the specific color of the counter of interest, and use analyze particles to count.

Michael Cammer, Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]    http://microscopynotes.com/
https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory

________________________________________
From: Flemming Ravn Merkel [[hidden email]]
Sent: Wednesday, October 25, 2017 6:46 PM
To: [hidden email]
Subject: Cell counter issue

Hi All

I use the Cell Counter plugin to mark and count seabirds on large composite images. Cell Counter automatically sum up all the different marker types for the entire image. However, occasionally I need to to sum up the markers for only a subarea of the image or to split up the total count according a grid overlay.

Any idea how to do this?

Kindly
Flemming


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[spt]

Yes, that works but if you pre-draw your ROI's and there aren't too many categories of cells to count, just using a different marker for each ROI/cell type combination is one less step

-----Original Message-----
From: Cammer, Michael [mailto:[hidden email]]
Sent: Wednesday, October 25, 2017 9:14 PM
To: [hidden email]
Subject: Re: Cell counter issue

Cell counter marks each criteria with different color.

Perhaps you could flatten the image thus stamping in the overlays, then draw ROI, segment the specific color of the counter of interest, and use analyze particles to count.

Michael Cammer, Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]    http://microscopynotes.com/
https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory

________________________________________
From: Flemming Ravn Merkel [[hidden email]]
Sent: Wednesday, October 25, 2017 6:46 PM
To: [hidden email]
Subject: Cell counter issue

Hi All

I use the Cell Counter plugin to mark and count seabirds on large composite images. Cell Counter automatically sum up all the different marker types for the entire image. However, occasionally I need to to sum up the markers for only a subarea of the image or to split up the total count according a grid overlay.

Any idea how to do this?

Kindly
Flemming


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