Cell length measurment

[Mehdi Nikkhah]

Dear all

 

I have grown cells on a flat substrate and I am trying to measure their
length,

If I want to do length measurement using Image J, is there anyway to bypass
one by one length measurement? I am trying to mark a line on the cells and
measure all the length at the same time, but it seems ImageJ does not have
the capability

 

I would appreciate if you could kindly let me know about this question  

 

 

Thanks

Mehdi Nikkhah

 

[Jacqueline Ross]

Hi Mehdi,

You just need to use the ROI Manager (Analyze - Tools - ROI Manager).
After you draw each line, just click on Add in the ROI Manager. When you
have all of the line selections added, you can then click on Measure in
the ROI Manager window. If you want to save the ROIs, then you can click
on Save and save the whole set.

Make sure when you go to measure, that you don't have any of the lines
highlighted in the ROI Manager. Otherwise, you will only get the
measurement for that individual line segment.

You should scale your image first so that you get the measurements in
the appropriate units.

Cheers,

Jacqui


Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Mehdi Nikkhah
Sent: Wednesday, 24 September 2008 5:00 a.m.
To: [hidden email]
Subject: Cell length measurment

Dear all

 

I have grown cells on a flat substrate and I am trying to measure their
length,

If I want to do length measurement using Image J, is there anyway to
bypass one by one length measurement? I am trying to mark a line on the
cells and measure all the length at the same time, but it seems ImageJ
does not have the capability

 

I would appreciate if you could kindly let me know about this question  

 

 

Thanks

Mehdi Nikkhah

 

[Mehdi Nikkhah]

Hi Jacqui

Thanks for your great description, it works perfectly

Regards
Mehdi

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Jacqui
Ross
Sent: Tuesday, September 23, 2008 10:12 PM
To: [hidden email]
Subject: Re: Cell length measurment

Hi Mehdi,

You just need to use the ROI Manager (Analyze - Tools - ROI Manager).
After you draw each line, just click on Add in the ROI Manager. When you
have all of the line selections added, you can then click on Measure in
the ROI Manager window. If you want to save the ROIs, then you can click
on Save and save the whole set.

Make sure when you go to measure, that you don't have any of the lines
highlighted in the ROI Manager. Otherwise, you will only get the
measurement for that individual line segment.

You should scale your image first so that you get the measurements in
the appropriate units.

Cheers,

Jacqui


Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Mehdi Nikkhah
Sent: Wednesday, 24 September 2008 5:00 a.m.
To: [hidden email]
Subject: Cell length measurment

Dear all

 

I have grown cells on a flat substrate and I am trying to measure their
length,

If I want to do length measurement using Image J, is there anyway to
bypass one by one length measurement? I am trying to mark a line on the
cells and measure all the length at the same time, but it seems ImageJ
does not have the capability

 

I would appreciate if you could kindly let me know about this question  

 

 

Thanks

Mehdi Nikkhah