Cell membrane detection and volume calculation

[Gary Stroup]

Hello,

  I am interested in using image j to detect the boundaries of the nuclear
and plasma membranes of cells, and from this calculate nuclear and cellular
volumes. Is there any code or plugins that can do this already? I would
greatly appreciate any help or guidance on this matter.

Gary

[jmarks]

What markers are you using to identify the plasma membrane and nuclear outline?

Gary Stroup wrote

  I am interested in using image j to detect the boundaries of the nuclear
and plasma membranes of cells

[monal9817]

hi, i am also interested in measuring the nuclear and cytoplasmic area, volume, perimeter and feret diameter using the imageJ software ca nany one explain the procedure or tell me which plugin can do this better ways

also want to know how to get individual cell value ( both Nuclear & Cytoplasm) ( area, perimeter,volume,feret diameter)

regard

Monal