Hi rmaeda,
From your description it sounds like you need segmentation, meaning the
fraction of cells labeled with Zenk that also have Hu, rather than
colocalization, which correlates Zenk and Hu labeling on a pixel-by-pixel
basis. If you are working with tissue culture, I think CellProfiler could
meet your needs with no problem. That is a free and open source program
designed specifically for that sort of segmentation problem. The
tutorials available on YouTube are quite excellent. There are many
segmentation protocols available for ImageJ/Fiji as well; I prefer
CellProfiler largely because it is purpose-built for the application and
quite user-friendly. Segmentation algorithms have a harder time with
tissue sections but I believe some have succeeded with that application as
well.
All the best,
Tim
Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology
On 3/15/16, 3:27 PM, "ImageJ Interest Group on behalf of rmaeda"
<
[hidden email] on behalf of
[hidden email]> wrote:
>Hello,
>
>I am currently trying to figure out a method to identify double-labeled
>BrdU/Zenk and triple-labeled BrdU/Zenk/Hu cells in my images. I found a
>plug
>in called "Just another colocalization protocol" and I have attempted to
>run
>it a couple times to identify double-labeled cells, but I cannot get it to
>work. Does anyone have experience with this Plug-in who could help? Or a
>suggested for another plug-in? Thank you!
>
>
>
>--
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>
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