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Hi,
I'm relatively new to ImageJ analysis and I was wondering what the best method for analysing nuclear translocation of transcription factors is. I have hundreds of files with separate colour channels for stained nuclei and stained transcription factor (TF).
At the very least, I'd like to be able to get a Manders' coefficient for overlap of one channel (TF) overlapping another channel (nuclei), but ideally I want to measure intensity of TF inside vs outside the nucleus on a single cell level.
Is there a way to write a macro that can call up each of my files individually(of which there are 4 separate tiled images in each file), split the channels, analyse the nuclear localisation, and store the output as text for every file without me having to go through them one at a time?
Thanks.
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