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Dear all,
Recently I have been trying the coloc 2 module and I have chosen ROI prior to the analysis. Regardless, the program spits out that I should use a ROI as the ration of y-intercept is far from zero. All my images have had background reduction.
Also, the thresholds for Ch1 and Ch2 are very high, which I guess originates in the ROI being too small to choose many more pixels.
I must make clear that the area I use for coloc is, however, very small, as it represents the small organelle of interest I am working on. Would this choice of such a small ROI bias the analysis this much? Should I then ignore the warnings and use the results?
Thanks
P
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