Colocalization - HELP!!!

[Tithi Dutta Roy]

Hi everyone,
I have used ImageJ in the past year to find cell area in images, that has
been great.  However, I now want to look at colocalization of adhesion
proteins in osteoblasts, and I am not quite sure what I get when I use the
various plugins.  I am most interested in the Colocalization Finder plugin,
as it seems to give some quantitative numbers on colocalization overlap,
but I am not sure what I am looking at.  HELP!!!  : )  If anyone has any
advice at all, that would be wonderful.  I am not signed up on the
listserv, so if you could just email me here at this address
([hidden email]), that would be great.  Thanks so much in advance,
and I am sure to get great advice from you guys.

Tithi

Tithi Dutta Roy, PhD
Postdoctoral Fellow, Biomaterials Group, Polymers Division
National Institute of Standards and Technology (NIST)
100 Bureau Drive, Mail Stop 8543
Building 224, Room A107
Gaithersburg, MD 20899-8543
Phone: [phone]
Fax: [phone]

[Ruy]

Hi

I am also interested in the Colocalization Finder plug-in.

I think it works fine for colocalization of double labeled fluorescent
samples.
 
Please, I would like to receive some input from the experts of this interest
group

Thanks

Ruy Jaeger

 ========================================================================
                   Dr. Ruy G. Jaeger, DDS, MSD, PhD

 University of Sao Paulo            Institute of Biomedical Sciences
             Department of Cell and Developmental Biology
 Av. Prof. Lineu Prestes 1524 sala 405            Sao Paulo SP 05508-900
 Phone    [phone]                         FAX    [phone]
                                BRAZIL
          website: http://www.icb.usp.br/~rgjaeger/index.html
 ========================================================================


On 13/9/05 4:06 PM, "Tithi Dutta Roy" <[hidden email]> wrote:

> Hi everyone,
> I have used ImageJ in the past year to find cell area in images, that has
> been great.  However, I now want to look at colocalization of adhesion
> proteins in osteoblasts, and I am not quite sure what I get when I use the
> various plugins.  I am most interested in the Colocalization Finder plugin,
> as it seems to give some quantitative numbers on colocalization overlap,
> but I am not sure what I am looking at.  HELP!!!  : )  If anyone has any
> advice at all, that would be wonderful.  I am not signed up on the
> listserv, so if you could just email me here at this address
> ([hidden email]), that would be great.  Thanks so much in advance,
> and I am sure to get great advice from you guys.
>
> Tithi
>
> Tithi Dutta Roy, PhD
> Postdoctoral Fellow, Biomaterials Group, Polymers Division
> National Institute of Standards and Technology (NIST)
> 100 Bureau Drive, Mail Stop 8543
> Building 224, Room A107
> Gaithersburg, MD 20899-8543
> Phone: [phone]
> Fax: [phone]
>

[Gabriel Landini]

On Tuesday 13 September 2005 20:39, Ruy wrote:
> I am also interested in the Colocalization Finder plug-in.
> I think it works fine for colocalization of double labeled fluorescent
> samples.
> Please, I would like to receive some input from the experts of this
> interest group

I would suggest to read the "More colocalization plugins..." thread in the
Mail archive.

Cheers,
G.