Color colocalization of 2 channels (Z-stacks) in ImageJ

[BrunoNP]

Hello everyone,

I have two z-stacks ("2 channels") and I need to quantify the number of colocalization points between the two z-stacks. I've only found plugins allowing me to analyze the full projection images of the z-stacks but I need to find the colocalization points while considering the 3D nature of my images.

Do you know any plugin in ImageJ that would allow me to this in either a 3D visual representation or just by comparing each image of each z-stack consecutively?
I would like to be able to visualize the colocalization points in case any manual correction may be needed.

Thank you in advance

[Thomas Boudier-3]

Hi,

You can try the 3DManager if you can segment your data, else the
classical Jacop should be helpful :

http://imagejdocu.tudor.lu/doku.php?id=plugin:stacks:3d_roi_manager:start

http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:jacop_2.0:just_another_colocalization_plugin:start

Best,

Thomas

On 21/09/2015 11:57, BrunoNP wrote:

> Hello everyone,
> I have two z-stacks ("2 channels") and I need to quantify the number of
> colocalization points between the two z-stacks. I've only found plugins
> allowing me to analyze the full projection images of the z-stacks but I need
> to find the colocalization points while considering the 3D nature of my
> images.
> Do you know any plugin in ImageJ that would allow me to this in either a 3D
> visual representation or just by comparing each image of each z-stack
> consecutively?
> I would like to be able to visualize the colocalization points in case any
> manual correction may be needed.
> Thank you in advance
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/Color-colocalization-of-2-channels-Z-stacks-in-ImageJ-tp5014383.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>
>
> --
>   /***************************************************************/
>        Thomas Boudier, Associate Professor, UPMC,
>        Université Pierre et Marie Curie, Paris, France.
>        BioInformatics Institute (BII)/IPAL, Singapore.
> /**************************************************************/

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