Color threshold selection

[cyclicAMP]

Dear esteemed list,

As many here I am relatively new to using ImageJ. I am currently analyzing DAB-stained lung sections where I want to select a ROI based on a color threshold. So far so good, however, the selection is applied to the whole image, whereas I only want to select a specific subset within this selection (only the region surrounding the airways). Could anyone help me with this problem?

[Djoere Gaublomme]

Hi cAMP,

What exactly do you want to do with the selection?

Sounds to me like you probably just want to draw a ROI manually  
(Freehand selections), then do 'Edit' - 'Clear outside', or  
alternatively copy your selection to a new picture.

grts

Djoere


Quoting cyclicAMP <[hidden email]>:

> Dear esteemed list,
>
> As many here I am relatively new to using ImageJ. I am currently analyzing
> DAB-stained lung sections where I want to select a ROI based on a color
> threshold. So far so good, however, the selection is applied to the whole
> image, whereas I only want to select a specific subset within this selection
> (only the region surrounding the airways). Could anyone help me with this
> problem?
>
>
>
> --
> View this message in context:  
> http://imagej.1557.x6.nabble.com/Color-threshold-selection-tp5010676.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[hesham977]

Perfect, but I think cyclicAMP needs steps for the whole process.

On Fri, Nov 28, 2014 at 5:59 PM, Djoere Gaublomme <[hidden email]
> wrote:

> Hi cAMP,
>
> What exactly do you want to do with the selection?
>
> Sounds to me like you probably just want to draw a ROI manually (Freehand
> selections), then do 'Edit' - 'Clear outside', or alternatively copy your
> selection to a new picture.
>
> grts
>
> Djoere
>
>
>
> Quoting cyclicAMP <[hidden email]>:
>
>  Dear esteemed list,
>>
>> As many here I am relatively new to using ImageJ. I am currently analyzing
>> DAB-stained lung sections where I want to select a ROI based on a color
>> threshold. So far so good, however, the selection is applied to the whole
>> image, whereas I only want to select a specific subset within this
>> selection
>> (only the region surrounding the airways). Could anyone help me with this
>> problem?
>>
>>
>>
>> --
>> View this message in context: http://imagej.1557.x6.nabble.
>> com/Color-threshold-selection-tp5010676.html
>> Sent from the ImageJ mailing list archive at Nabble.com.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
*Dr. Hesham Noaman Abdelraheem Mustafa*
Assistant Professor, Faculty of Medicine, King Abdulaziz University,
ResearcherID: http://www.researcherid.com/rid/H-5618-2011
ORCID: http://orcid.org/0000-0003-1188-2187
Address: P.O.Box 80205   Jeddah, 21589   Saudi Arabia.
Phone: (Office):  [phone] Ext. 22016
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[cyclicAMP]

I want only to measure the surface area of the (desired) selection. Removing unwanted regions of the selection with the Freehand selection tool (holding the ALT key) seems to do the trick. I wish there would be a faster method, but this will do for now I guess. Thanks for the suggestion!