Confocal Data For Geometric Morphometric Analysis

[Chris Rhodes]

Hello

Geometric morphometrics involves analysis of shape changes based upon data
from homologous landmarks (corresponding anatomical points from specimens).
I therefore need to obtain the 3D coordinates of specific parts of the image
in the most efficient manner possible.

I've seen discussion of saving X,Y coordinates in the archive, but I need to
save X,Y,Z coordinates if possible.

I have looked at the program, but I might well be missing something that is
obvious to the experienced user.

I have already used the BLJ plugin for volume/surface rendering of Z-stacks
".lsm" files in 3D. I now need to be able to collect quantitative data from
the file.

Thank You

Chris


----Original Message Follows----
From: vytas <[hidden email]>
To: [hidden email]
Subject: Re: Morphometrics On Confocal Data
Date: Thu, 04 Aug 2005 09:45:10 -0500


>Hello Chris,

         Re your posting to ImageJ list, perhaps you should be more explicit
about the type of measurements/data you wish to extract and the type of
data.
         regards,


Vytas Bindokas, Ph.D.
Research Assoc. / Assoc. Prof.,
Director, BSD Light Microscopy Core Facility
Dept Neurobiol Pharmacol Physiol MC0926
947 E 58th Street
The University of Chicago
Chicago IL 60637
Room J246A (SBRI)

773-834-9040
fax [phone]
email [hidden email]
  web site for LMCF:
http://digital.bsd.uchicago.edu/index.html

----------------------------------------------------------------------------------------------------------------------

Hello

What is the best way to collect 3D landmark data from Z-stacks?

I want to collect data using a Zeiss confocal laser scanning microscope,
which produces ".lsm" files, and perform 3D geometric morphometrics on it.

Please let me know if other software could enhance this process.

Chris Rhodes