If anyone is interested in a solution for creating consistent images over
multiple microscope sessions using color brightfield images, a free webinar
is coming up. The beauty of consistent images is that a single threshold
can be used, removing user subjectivity when setting threshold manually,
and software errors using auto-threshold. Because the solution involves a
commercial product, please write to me at
[hidden email] if you
would like the time and date of the free webinar.
On another note, I have been finding a disconcerting phenomenon in
approximately 50% of the labs that I've visited: images created via color
camera systems are non-linear. With this 50%, I found that software used
with color cameras apply a non-linear gamma.
If scientists are using non-linear images for optical density
quantification without calibrating to a standard, the data is false, and,
in addition, the images are misleading (for qualitative analysis).
I would expect a default setting that produces a linear image, and
user-intervention methods that would then create non-linear images. I
found that it was difficult, and in some cases impossible, to create a
linear image from this 50% of camera systems.
I would encourage participation in the free webinar if only to learn more
about the linearity issue and its impact on data and published images.
Best,
Jerry Sedgewick
--
Jerry (Gerald) Sedgewick
Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output"
Automated Image Measurement services and systems; On-Site and on-line
training for image acquisition/processing/quantitation in science,
microscopy and medicine.
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html
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