Convert tif file to lif file

[marykef]

Hi,

I would like to ask how to convert a tif file to a lif file?

Thank you in advance




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[Curtis Rueden-2]

Hi,

> I would like to ask how to convert a tif file to a lif file?

To my knowledge, no one has coded a Leica LIF writer for ImageJ. Leica LIF
is a proprietary image file format (PFF), which can be read by the
Bio-Formats library. The development philosophy though is to support only
reading, not writing, of PFFs, in favor of writing to open file formats
such as ICS and OME-TIFF, to encourage interoperability of data via open
standards.

In general, to convert between formats, you can use the bfconvert command
line tool:

https://docs.openmicroscopy.org/bio-formats/5.7.3/users/comlinetools/conversion.html

Or one of ImageJ's export plugins; see:
* https://imagej.net/Category:Import-Export
* https://imagej.net/ij/docs/guide/146-7.html#toc-Section-7
* https://imagej.net/ij/plugins/#io

Regards,
Curtis

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Curtis Rueden
Software architect, LOCI/Eliceiri lab - https://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - https://imagej.net/User:Rueden
Have you tried the Image.sc Forum? https://forum.image.sc/



On Thu, Jun 25, 2020 at 1:19 PM marykef <[hidden email]> wrote:

> Hi,
>
> I would like to ask how to convert a tif file to a lif file?
>
> Thank you in advance
>
>
>
>
> --
> Sent from: http://imagej.1557.x6.nabble.com/
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Schebique]

Hi.

If you really need the .lif image format, I think you can use Las X lite
<https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/>
to import tiff files and save them as one .lif file.

Best, Ondrej.

čt 25. 6. 2020 v 21:00 odesílatel Curtis Rueden <[hidden email]>
napsal:

> Hi,
>
> > I would like to ask how to convert a tif file to a lif file?
>
> To my knowledge, no one has coded a Leica LIF writer for ImageJ. Leica LIF
> is a proprietary image file format (PFF), which can be read by the
> Bio-Formats library. The development philosophy though is to support only
> reading, not writing, of PFFs, in favor of writing to open file formats
> such as ICS and OME-TIFF, to encourage interoperability of data via open
> standards.
>
> In general, to convert between formats, you can use the bfconvert command
> line tool:
>
>
> https://docs.openmicroscopy.org/bio-formats/5.7.3/users/comlinetools/conversion.html
>
> Or one of ImageJ's export plugins; see:
> * https://imagej.net/Category:Import-Export
> * https://imagej.net/ij/docs/guide/146-7.html#toc-Section-7
> * https://imagej.net/ij/plugins/#io
>
> Regards,
> Curtis
>
> --
> Curtis Rueden
> Software architect, LOCI/Eliceiri lab - https://loci.wisc.edu/software
> ImageJ2 <https://loci.wisc.edu/softwareImageJ2> lead, Fiji maintainer -
> https://imagej.net/User:Rueden
> Have you tried the Image.sc Forum? https://forum.image.sc/
>
>
>
> On Thu, Jun 25, 2020 at 1:19 PM marykef <[hidden email]> wrote:
>
> > Hi,
> >
> > I would like to ask how to convert a tif file to a lif file?
> >
> > Thank you in advance
> >
> >
> >
> >
> > --
> > Sent from: http://imagej.1557.x6.nabble.com/
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Mgr. Ondřej Šebesta
Laboratory of Confocal and Fluorescence Microscopy
Faculty of Science, Charles University in Prague
Vinicna 7
128 44 Prague
Czech Republic

Phone: [phone]
e-mail: [hidden email]

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