Coordinates of the center of a particle in a densly packed image

[Pralay Kanti Santra]

Hi,

I am trying to find out the coordinates of a densely packed monodisperse
particle. When I move the cursor after loading the image in Imaje J, it
shows me the coordinates. Is these any way to write the coordinate in a file
after clicking on the center of the particle? Can you please help me out in
this regard?

I have tried to do the same by analyzing the center of mass as well as
centroid but it did not work (I think because of very densely packed
particles!).

Thank you in advance.

With regards,

Pralay Kanti Santra.

[Wayne Rasband]

> Hi,
>
> I am trying to find out the coordinates of a densely packed
> monodisperse
> particle. When I move the cursor after loading the image in Imaje J, it
> shows me the coordinates. Is these any way to write the coordinate in
> a file
> after clicking on the center of the particle? Can you please help me
> out in
> this regard?
>
> I have tried to do the same by analyzing the center of mass as well as
> centroid but it did not work (I think because of very densely packed
> particles!).

The point tool records coordinates in the Results table if you enable
"Auto-Measure" in the Edit>Options>Point Tool dialog. You can open this
dialog by double clicking on the point tool, which has a cross-hair
icon. To save the recorded coordinates in a file, right-click in the
Results window and select "Save As..." from the drop down menu.

-wayne

[Pralay Kanti Santra]

Hi Wayne,

Many many thanks for the information.

Is there any way to mark the place where I am measuring the coordinates?

With regards,

Pralay.

On Mon, Nov 17, 2008 at 11:56 PM, Wayne Rasband <[hidden email]> wrote:

> Hi,
>>
>> I am trying to find out the coordinates of a densely packed monodisperse
>> particle. When I move the cursor after loading the image in Imaje J, it
>> shows me the coordinates. Is these any way to write the coordinate in a
>> file
>> after clicking on the center of the particle? Can you please help me out
>> in
>> this regard?
>>
>> I have tried to do the same by analyzing the center of mass as well as
>> centroid but it did not work (I think because of very densely packed
>> particles!).
>>
>
> The point tool records coordinates in the Results table if you enable
> "Auto-Measure" in the Edit>Options>Point Tool dialog. You can open this
> dialog by double clicking on the point tool, which has a cross-hair icon. To
> save the recorded coordinates in a file, right-click in the Results window
> and select "Save As..." from the drop down menu.
>
> -wayne
>

--
Research Student
Solid State and Structural Chemistry Unit
Indian Institute of Science
Bangalore 560 012 INDIA

Ph No: [phone] (Extn: 2052)
Fax No: [phone]
Mob No: [phone]

[W. Chan]

cell counter plugin

http://rsb.info.nih.gov/ij/plugins/cell-counter.html

On Tue, 18 Nov 2008, Pralay Kanti Santra wrote:

> Hi Wayne,
>
> Many many thanks for the information.
>
> Is there any way to mark the place where I am measuring the coordinates?
>
> With regards,
>
> Pralay.
>
> On Mon, Nov 17, 2008 at 11:56 PM, Wayne Rasband <[hidden email]> wrote:
>
> > Hi,
> >>
> >> I am trying to find out the coordinates of a densely packed monodisperse
> >> particle. When I move the cursor after loading the image in Imaje J, it
> >> shows me the coordinates. Is these any way to write the coordinate in a
> >> file
> >> after clicking on the center of the particle? Can you please help me out
> >> in
> >> this regard?
> >>
> >> I have tried to do the same by analyzing the center of mass as well as
> >> centroid but it did not work (I think because of very densely packed
> >> particles!).
> >>
> >
> > The point tool records coordinates in the Results table if you enable
> > "Auto-Measure" in the Edit>Options>Point Tool dialog. You can open this
> > dialog by double clicking on the point tool, which has a cross-hair icon. To
> > save the recorded coordinates in a file, right-click in the Results window
> > and select "Save As..." from the drop down menu.

--
Pang (Wai Pang Chan, [hidden email], PAB A087, 206-685-1519)
The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)