You could try the following.
Microscopy with a low NA lens to capture entire depth of cell in one image.
Measure area & mean intensity of each cell, area * intensity.
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
[hidden email] http://nyulmc.org/micros http://microscopynotes.com/
Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Chris O'Connell
Sent: Friday, August 10, 2018 9:30 AM
To:
[hidden email]
Subject: Correcting Intensity Measurements for Cell Rounding
Hi All,
We want to plot integrated fluorescent intensity over time in cultured
cells. The cells are rounding during the recording. Is there a generally
accepted way to correct for this using the area of the cells?
Thanks in advance.
Chris O'Connell
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