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Hi there,
I am measuring the distance between two red and green gaussian fitted curves. I then repeat on more fields to get an N= aprox 500 and calculate an average (I am using a macro to automate this).
I think that the red and green pixel offest is increasing the standard deviation of the calculated average. I would like to measure the offest (presumably using beads that flouresce in both channels), and then re-run a modified version of the macro that corrects for it.
Are there any macros out there already that do something similar that I might be able to adapt?
Can anyone suggest a good route forward? I am new to writing macros, and not yet very good at it, so help would be gratefully received.
Many thanks,
Sam
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