Counting cell and mean grey

[JoeML]

Hello everyone

I have a doubt about If I can count cell and mean grey of each cell at the same time in a picture. I know the first  step it is use a threshold and with the tool "analyze particules" try to get that parameters, but i am not sure the way to use it. Add a picture.

 

Thank you very much for the help

[Brioso Jimenez, Jose]

You have to go to analyze>set measurements/ and there select Area, Mean gray value, Integrated density.

When you use analyze particles you will have to select a treshold instead of from 0 to infinite, change the value 0 for a value of area next to the one of your cells, so it recognizes only the shape of them. After that they will be selected (with analyze particles), and then you will have acces to ROI manager, there you select all your cells and click in measure and you will get the data you are looking for.

________________________________________
De: ImageJ Interest Group <[hidden email]> en nombre de JoeML <[hidden email]>
Enviado: lunes, 9 de marzo de 2015 16:38
Para: [hidden email]
Asunto: Counting cell and mean grey

Hello everyone

I have a doubt about If I can count cell and mean grey of each cell at the
same time in a picture. I know the first  step it is use a threshold and
with the tool "analyze particules" try to get that parameters, but i am not
sure the way to use it. Add a picture.

  <http://imagej.1557.x6.nabble.com/file/n5011925/Foto_6.jpg>

Thank you very much for the help



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