Counting fliuorescene nuclei

[ich_all]

Dear,

I have an experiment with NLS::GFP construct. The positive result is the fluorescence nuclei with black backgroud. I would like to quantify them using ImageJ but I don't know how to do it. I will really appreciate if you have solutions for me. I attach one file for an example

PS: I am a mac user

 

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Ahmad Faizal
PhD Student
Department of Plant Production, Faculty of Bioscience Engineering, Ghent University
Coupure Links 653, 9000 Gent, Belgium
Office: A100.060
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Ontmoeting Buitelandse Studenten Gent (OBSG)
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[wangleicau]

I am not sure my way works for you or it is correct, but that what I do in my lab to score IF images:
   1. Go to PROCESS> BINARY or IMAGE>TYPE>8BIT
   2. Go to IMAGE>ADJSUT>THRESHOLD to set up lower and upper threshold
   3. Use ANALYZE > MEZSURE to know the pixels for different shapes of AIM to count
   4. Go to ANALYZE > ANALYZE PARTICLES and the window