Counting points within a specified area

[Sinead Roberts]

Dear all,

I would like to draw an ROI and then to count points within that ROI  
that I have manually marked within the ROI (I basically have an image  
where I am counting the frequency of organelles, but it is an electron  
microscopy image and not possible to threshold the image in such a way  
to automatically count the organelles of interest); is there a way to  
measure the area of the ROI and the number of marks I have made within  
that area within ImageJ? Or will I have to measure the area and then  
manually count the number of marks I have made and record that number  
myself independently of ImageJ?

Thanks very much in advance for your help,
Sinead

[Daniel Lélis Baggio]

Hi,
how have you marked the points in the ROI?
Is it through a selection, a binary image, painting, how was that?

Thanks,
Daniel

On Thu, May 6, 2010 at 7:34 AM, sinead roberts <[hidden email]> wrote:

> Dear all,
>
> I would like to draw an ROI and then to count points within that ROI that I
> have manually marked within the ROI (I basically have an image where I am
> counting the frequency of organelles, but it is an electron microscopy image
> and not possible to threshold the image in such a way to automatically count
> the organelles of interest); is there a way to measure the area of the ROI
> and the number of marks I have made within that area within ImageJ? Or will
> I have to measure the area and then manually count the number of marks I
> have made and record that number myself independently of ImageJ?
>
> Thanks very much in advance for your help,
> Sinead
>