Custom TEM acquisition software

[Toby Cornish]

Has anyone on this list written custom acquisition software for a TEM-compatible digital camera?

I have been consulted on this topic by our TEM facility and Informatics Division, but my personal experience is in fluorescence digital photomicrography, not TEM-compatible digital cameras.

As ImageJ is my preferred library for image processing, I am curious if anyone here has experience interfacing with a TEM digital camera.

thanks,

Toby



Toby C. Cornish, M.D., Ph.D.
Pathology Resident
Johns Hopkins Medical Institutions
[hidden email]

[Albert Cardona]

Hi Toby,

Best software I know (and open source!) is Leginon, made by the
http://ami.scripps.edu people. It's made in python and interfaces a
variety of FEI microscopes, and Gatan and Tietz cameras.

I use it extensively. Then I process the images with ImageJ + TrakEM2,
the latter an ImageJ plugin of my own cooking available here:

    http://www.ini.uzh.ch/~acardona/trakem2.html

You can see some of the results of combined Leginon and TrakEM2 here:

    http://fly.mpi-cbg.de

Just choose the "Leginon" slice, which was generated with automatic tile
stitching, histogram correction and background removal options.

Albert

--
Albert Cardona
http://www.mcdb.ucla.edu/Research/Hartenstein/acardona