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DAB quantification using imageJ The usual question

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hesham977

DAB quantification using imageJ The usual question

Dear All
I need an urgent help
The usual question about DAB quantification using imageJ
I have renal specimens and I used Desmin and caspase-3 immunohistochemistry
markers.
I'm in-need to measure area percentage and optical density if possible
*can some one help me with clear steps*

I think about imageJ, Fuji and Immunohistochemistry (IHC) Image Analysis
Toolbox

Waiting for your urgent contribution.

--

*Dr. Hesham Noaman Abdelraheem MustafaAssistant Professor,Faculty of
Medicine, King Abdulaziz University,e-Library Assistant,Student Support
Unit Member,Nutrition & Metabolism Module Coordinator.Address: P.O.Box
80205 Jeddah, 21589 Saudi
Arabia.Phone: (Office): +966 2 6400000 Ext. 22016
(Mobile): +966 566 764 762 (Egypt): +20 1001 460 009*

*Official Email: [hidden email] <[hidden email]>*

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

Dr. Hesham N Mustafa Professor, Faculty of Medicine, King Abdulaziz University, ResearcherID: http://www.researcherid.com/rid/H-5618-2011 ORCID: http://orcid.org/0000-0003-1188-2187 Address: P.O.Box 80205 Jeddah, 21589 Saudi Arabia. Phone: (Office): +966 2 6400000 Ext. 22016 (Mobile): +966 566 764 762 (Egypt): +20 1001 460 009 Official Email: hmustafa@kau.edu.sa

ctrueden

Re: DAB quantification using imageJ The usual question

Hi Hesham,

> I'm in-need to measure area percentage and optical density if possible
> *can some one help me with clear steps*

Start here:
http://imagej.net/Segmentation

And see also:
http://www.researchgate.net/post/Any_good_methods_for_staining_quantifications_using_computer_programs

Regards,
Curtis

On Tue, Nov 4, 2014 at 12:10 PM, Dr. Hesham Noaman Abdelraheem Mustafa <
[hidden email]> wrote:

> Dear All
> I need an urgent help
> The usual question about DAB quantification using imageJ
> I have renal specimens and I used Desmin and caspase-3 immunohistochemistry
> markers.
> I'm in-need to measure area percentage and optical density if possible
> *can some one help me with clear steps*
>
> I think about imageJ, Fuji and Immunohistochemistry (IHC) Image Analysis
> Toolbox
>
> Waiting for your urgent contribution.
>
> --
>
>
>
>
>
>
>
>
>
>
>
>
>
> *Dr. Hesham Noaman Abdelraheem MustafaAssistant Professor,Faculty of
> Medicine, King Abdulaziz University,e-Library Assistant,Student Support
> Unit Member,Nutrition & Metabolism Module Coordinator.Address: P.O.Box
> 80205 Jeddah, 21589 Saudi
> Arabia.Phone: (Office): +966 2 6400000 Ext. 22016
> (Mobile): +966 566 764 762 (Egypt): +20 1001 460 009*
>
> *Official Email: [hidden email] <[hidden email]>*
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html