Thanks Wolfgang!
I guess you're right any method should work as long as I treat my
standards the same. I will give several methods a try and see which one
gives me the most reproducible data.
Best wishes,
Cordy
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Wolfgang Schechinger
Sent: 31 May 2007 15:13
To:
[hidden email]
Subject: Re: DNA Quantification
Hi Cordy,
I think the gel analysis menu (strg 1/2/3) is the right choice.
As you probably want a standard curve / "line", it might be necessary to
convert your image to some sort of pseudo OD, from ij1.38o, there is a
nice function in the calibrate menu - thanks Wayne!.
As you have your standards in the scan, actually every method should do
it, as long as you treat your standards and the samples the same.
Best regards,
Wolfgang
-------- Original-Nachricht --------
Datum: Wed, 30 May 2007 09:49:27 -0400
Von: Cordy <
[hidden email]>
An:
[hidden email]
Betreff: DNA Quantification
> Hi, I was wondering if anyone could help me. I am trying to quantify
ng of
> DNA using my ladder as standard. Should i be using the area under the
plot
> >from the gel analysis menu or the profile plots?
>
> Any help you could provide would be greatly appreciated.
>
> Best wishes,
>
> Cordy
--
GMX FreeMail: 1 GB Postfach, 5 E-Mail-Adressen, 10 Free SMS.
Alle Infos und kostenlose Anmeldung:
http://www.gmx.net/de/go/freemail
Locked
