Hi Thorsten
In deconvolution software the PSF and image have to eventually be the same
size with the same z-spacing so that they can be transformed to FFT space
for calculations.
In many programs this is done internally through a combination of
resampling and padding. In deconvolutionlab I don't think it resamples the
PSF based on z voxel size.
So yes I think you would have to use a PSF and image with the same z
spacing.
If you want to capture an image with different z-spacing you could try
rescaling the PSF to match the image z spacing.
On Tue, May 27, 2014 at 8:25 AM, Thorsten Wagner <
[hidden email]> wrote:
> Hi,
>
> I currently try to deconvolve an image stack of a macrophage captured
> with dark field microscopy.
>
> I've measured the PSF using using nanoparticles with a diameter of
> 150nm. I captured slices of that PSF with a z-distance of 180nm (-5µm to
> 5µm from the focal plane).
>
> Now I want to use DeconvolutionLab to deconvolve my images.
> Just to be sure: The image stack which has to be deconvolved also have
> to provide a z-distance of 180nm? Or, to put the question in a different
> way: If I want use DeconvolutionLab, the z-distance of the PSF and of
> the input image have to be the equal?
>
> Cheers,
> Thorsten
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html
Locked
