Densitometry guide (lines)?

[Wolfgang Schechinger]

Dear IJ experts,

we use ImageJ for the densitometry of western blots (ctrl 1/2/3
sequece), raw data is generated by an infrared fluorescence scanner
(Licor Odyssey). Are there any guidelines how to define peaks and to
integrate them, esp. when there is no baseline separation of bands or
the baseline is sort of skew?

Are there sort of "official rules", articles one should know or a
good book (chapter) on the topic?
Googling was not much successful for me yet.

Many thanks and best regards,

Wolfgang

[jharford]

"Are there any guidelines how to define peaks and to integrate them, esp. when there is no baseline separation of bands or the baseline is sort of skew?"

Another possible solution would be to use LI-COR's free Image Studio Lite software (www.licor.com/islite) to analyze your western blot data from the Odyssey.  They have quickstart guides, tutorials and videos on how to define bands and integrate them, but it is pretty easy to use.